| 出品公司: | Promega |
|---|---|
| 载体名称: | pBiT1.1-C[TK/LgBiT] |
| 载体又名: | pBiT1.1-C[TK/LgBiT]载体，pBiT1.1-C[TK/LgBiT]质粒，pBiT1.1-C[TK/LgBiT]载体质粒 |
| 英文名称: | pBiT1.1-C[TK/LgBiT], pBiT1.1-C[TK/LgBiT] Vector, pBiT1.1-C[TK/LgBiT] Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | HSV TK |
| 载体大小: | 3.9 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | |
| 产品目录号: | N20242172 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
pBiT1.1-C[TK/LgBiT]
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pBiT1.1-C[TK/LgBiT]载体质粒基本信息
pBiT1.1-C[TK/LgBiT]质粒图谱载体图谱和pBiT1.1-C[TK/LgBiT]载体序列质粒序列多克隆位点信息
![pBiT1.1-C[TK/LgBiT]载体图谱](/uploads/载体图谱/pBiT1.1-C[TK-LgBiT]载体图谱.png)
![pBiT1.1-C [TK/LgBiT]多克隆位点](/uploads/载体图谱/pBiT1.1-C [TK-LgBiT]-MCS.png "pBiT1.1-C [TK/LgBiT]载体多克隆位点")
pBiT1.1-C[TK/LgBiT]质粒载体简介
pBiT1.1-C[TK/LgBiT]质粒序列载体序列
Snapgene载体序列下载: pBiT1.1-C[TK-LgBiT]序列 .gb格式载体序列下载:pBiT1.1-C[TK-LgBiT]载体序列
LOCUS pBiT1.1-C[TK-LgBiT] 3865 bp DNA circular SYN 19-JUL-2026
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3865)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 8.1.0 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1135209600|
COMMENT VNTDBDATE|1135209600|
COMMENT VNTNAME|pBiT1.1-C[TK-LgBiT]|
COMMENT VNTAUTHORNAME|DotmaticsRD|
FEATURES Location/Qualifiers
promoter 28..779
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
polyA_signal 1419..1540
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1959..2547)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(join(2747..3538,3539..3607))
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
polyA_signal 3712..3760
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 3774..3865
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagtctaaat gagtcttcgg acctcgcggg ggccgcttaa
61 gcggtggtta gggtttgtct gacgcggggg gagggggaag gaacgaaaca ctctcattcg
121 gaggcggctc ggggtttggt cttggtggcc acgggcacgc agaagagcgc cgcgatcctc
181 ttaagcaccc ccccgccctc cgtggaggcg ggggtttggt cggcgggtgg taactggcgg
241 gccgctgact cgggcgggtc gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac
301 ccccgggcgg cgccgccgcg gcggcgacgg gctcgctggg tcctaggctc catggggacc
361 gtatacgtgg acaggctctg gagcatccgc acgactgcgg tgatattacc ggagaccttc
421 tgcgggacga gccgggtcac gcggctgacg cggagcgtcc gttgggcgac aaacaccagg
481 acggggcaca ggtacactat cttgtcaccc ggaggcgcga gggactgcag gagcttcagg
541 gagtggcgca gctgcttcat ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg
601 gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc cagcgtcttg
661 tcattggcga agtcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
721 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga cccgcttaaa
781 agcttggcaa tccggtactg ttggtaaagc caccagatct gctagcgatc gcctaagtgg
841 gagctcaggg gaattctggc tcgagcggtg gtggcgggag cggaggtgga gggtcgtcag
901 gtgtcttcac actcgaagat ttcgttgggg actgggaaca gacagccgcc tacaacctgg
961 accaagtcct tgaacaggga ggtgtgtcca gtttgctgca gaatctcgcc gtgtccgtaa
1021 ctccgatcca aaggattgtc cggagcggtg aaaatgccct gaagatcgac atccatgtca
1081 tcatcccgta tgaaggtctg agcgccgacc aaatggccca gatcgaagag gtgtttaagg
1141 tggtgtaccc tgtggatgat catcacttta aggtgatcct gccctatggc acactggtaa
1201 tcgacggggt tacgccgaac atgctgaact atttcggacg gccgtatgaa ggcatcgccg
1261 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg
1321 acgagcgcct gatcaccccc gacggctcca tgctgttccg agtaaccatc aacagctaat
1381 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
1441 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
1501 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
1561 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
1621 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
1681 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
1741 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
1801 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
1861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
1921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
1981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
2041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
2101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
2161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
2221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
2281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
2341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
2401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
2461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
2521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
2581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
2641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
2701 taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga
2761 tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc
2821 ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga
2881 tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga
2941 gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct
3001 gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg
3061 ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc
3121 agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag
3181 ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag
3241 cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt
3301 actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt
3361 ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc
3421 gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc
3481 ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg
3541 caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga
3601 tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct
3661 ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata
3721 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
3781 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
3841 agtgcaggtg ccagaacatt tctct
//
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